Part:BBa_K4759011

GFP11

When gfp10 and gfp11 were fused to the target proteins a and b, respectively, and then the two fusion proteins were coexpressed in gfp1-9, due to the affinity between gfp1-9 and gfp11, when gfp10 and gfp11 interact through the target protein, gfp1-9 was also recruited nearby because of the affinity with gfp11, and the three segments were repooled to restore fluorescence activity. This tripartite sf gf p system can be used for protein interaction detection. In addition to the intuitive advantages, the gfp10 and gfp11 are both short peptides, so the influence on the fusion protein structure is probably small. In addition, because it is divided into three parts, the fluorescence background is further reduced, which helps to improve the signal-to-noise ratio of the detection.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]